181. Mysterious protein degradation - (reply: 4)
182. Purification granulocyte factor - (reply: 2)
183. Checking recombinant protein size - (reply: 3)
184. How to extract Large protein from Low percentage SDS-Glycine-PAGE gel? - (reply: 1)
185. Problems with Immunoprecipitation(Cross-linking Ig) - (reply: 3)
186. Protein Isolation of very low cell number... - (reply: 4)
187. Flag tag not working, although theoretically it's there - (reply: 1)
188. Protein extraction from periplasmic space - a simple protocol anyone? (reply: 1)
189. HELP:protein over-expression failure?! - (reply: 1)
190. membrane protein extraction - membrane protein extraction (reply: 7)
191. half life time of GFP? - (reply: 1)
192. protein band is shifted after boiling sample with sample buffer - (reply: 7)
193. Reverse phase chromatography of acetone protein precipitate - (reply: 2)
194. frezzing sample and Co-IP - ask a question (reply: 5)
195. Trouble expressing 155 kDa protein in pET21a - (reply: 3)
196. DNAse I incubation - shortest sufficient time - (reply: 9)
197. protein extraction - (reply: 4)
198. Protein questions - (reply: 6)
199. Cell fractionation problem - Nucleaus forms a pellet that can't be suspended (reply: 5)
200. Dialysis Buffer for Recombinant Protein - (reply: 5)
201. 2D gels.. comparison software? - (reply: 1)
202. 2D Electrophoresis for protein inclusion bodies/lysate? - sample preparation? (reply: 1)
203. protein recovery problems in analytical size exclusion chromatogrphay - or how do i have a more sensitive method? (reply: 3)
204. buffers effect on size exclusion chromatography.. - amm bicarb, phospate with or without salt/ethanol. (reply: 2)
205. to explain why i see two forms the same protein in SDS-PAGE - (reply: 1)
206. phytase enzyme assay - (reply: 6)
207. collagen labelling - (reply: 1)
208. Using 4-parameter fit for colorimetric assays for protein concentration? - how appropriate is it.. higher concentraion.. no linearity? (reply: 1)
209. Non specific bands on western blot. - (reply: 2)
210. PABP Inhibitor - (reply: 2)

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