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Protein-and-Proteomics
1411.
I want to strip the protein off PVDF membrane -
(reply: 4)
1412.
using two primary antibodies for WB? -
(reply: 3)
1413.
Maybe dumb question - MgCl2 lysis buffer -
(reply: 6)
1414.
Western Blot - unspecific antibody binding -
(reply: 9)
1415.
Problem with EGFP in translation study -
(reply: 12)
1416.
LOW concentration protein assay - help!
(reply: 5)
1417.
reducing background in western... - ...by blocking the antibodies
(reply: 2)
1418.
stripping western blot -
(reply: 3)
1419.
Protocol for reuse of PD-10 desalting columns -
(reply: 1)
1420.
Periplasmic protein extraction problem! -
(reply: 2)
1421.
SDS page gel GST beads or not to? -
(reply: 3)
1422.
Protein sample preparation for SDS-PAGE -
(reply: 9)
1423.
HELP! PAGE Gel Running problem -
(reply: 9)
1424.
Help with protein extraction! - WI-38 cells
(reply: 1)
1425.
Protein expression - decreased expression
(reply: 2)
1426.
western - cells treated w/ protein synethesis inhibitor -
(reply: 1)
1427.
Reducing protein-protein interaction -
(reply: 1)
1428.
so how to cleave off the GST? -
(reply: 11)
1429.
Immunoprecipitation in Non-reducing conditions? - Protocol suggestions?
(reply: 2)
1430.
Can I simply add ECL again? -
(reply: 2)
1431.
microgram to micromolar conversion - Protein concentration - microgram to micromolar conversion - Protein concentration
(reply: 4)
1432.
direct / indirect detection in WB -
(reply: 3)
1433.
beta-actin in serum? -
(reply: 4)
1434.
How to lyse plant nuclei? and keep nuclear protein complex stable -
(reply: 1)
1435.
How to normalize the protein expression amongst different tissues -
(reply: 7)
1436.
cancer-specific proteins -
(reply: 1)
1437.
Maltose Binding Fusion Protein MBP - which strain for expression?
(reply: 1)
1438.
Unknown receptor identification... -
(reply: 3)
1439.
A SPR question with Biacore -
(reply: 1)
1440.
can we purify the protein from westernblot membrane? -
(reply: 5)
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