1951. western blot ECL vs fluorescence - (reply: 2)
1952. How would you increase separation on PAGE? - (reply: 16)
1953. Tricine SDS-PAGE gel under non-reducing - please tell me a protocol (reply: 1)
1954. problems in concentration protein solution - (reply: 8)
1955. What is the best assay for protein interaction analysis? - (reply: 1)
1956. Gst tag purification problem - (reply: 5)
1957. Gel permeation & filtration - difference plz? (reply: 1)
1958. Question about proteinase cleavage sites - (reply: 1)
1959. Protease Inhibitors - (reply: 8)
1960. problem with immunoprecipitation - (reply: 1)
1961. is there any problem if the fusion protein was used directly as the antigen? - fusion protein was used directly as the antigen (reply: 4)
1962. what is the molecular weight of fusion protein in pET-32a - molecular weight of the fusion protein in pET-32a (reply: 3)
1963. FLAG M2 affinity gel agarose storage condition - (reply: 1)
1964. what makes a protein insoluble? - (reply: 3)
1965. Pellet after centrifuging - (reply: 2)
1966. Concentration of secreted protein - ...step before IP.... (reply: 1)
1967. Pellet the proteins!? - (reply: 4)
1968. Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
1969. Loading control - (reply: 3)
1970. normalizing for loading and transfer error during western blot - (reply: 4)
1971. Protein determination in a insoluble pellet - (reply: 6)
1972. Protein purification wash problem - (reply: 1)
1973. protein storage following purification - (reply: 1)
1974. Expression temprature - (reply: 3)
1975. urea/glycerol PAGE and cutting out the band... - cant find out protocols (reply: 1)
1976. protein elution from PAA gels - (reply: 2)
1977. Immunoaffinity chromatography-help - protocol for coupling of antibodies (reply: 2)
1978. Problem in protein preparation in 2-D electrophoresis - (reply: 1)
1979. database search for MALDI peptide peaks - Protein identification....help needed (reply: 4)
1980. does high concentration of Urea break down oligomers? - (reply: 3)

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