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481. Sponge pads in semi-dry westernblotting? - to save Whatman paper... (reply: 3)
482. cytosolic and nucleus fractionation protocol plz - (reply: 2)
483. WB anti-caveoline1 not reproducible - (reply: 2)
484. inclusion bodies - (reply: 1)
485. How to correct the delay from detection to colletion in rpHPLC? - (reply: 8)
486. Native acrylamid gel - (reply: 10)
487. Western for membrane bound protein - (reply: 11)
488. Pierce ECL femto kit - loses effectiveness? (reply: 1)
489. Biotin EMSA Blocking buffer - Search for a suitable blocking buffer (reply: 1)
490. Help for sample preparation for 2-D - How can I get the protein for IEF (reply: 1)
491. who can help me with staining with 80kD to 130KD protein family? - it is very difficult for me to successfully staining with integrin (reply: 14)
492. Identification of monomers/dimers in whole cell lysate via SDS-PAGE - (reply: 3)
493. IPG strip burned - (reply: 2)
494. Too many problems in 2D - estimation? staining? running? (reply: 4)
495. ECL signal dies off rapidly - (reply: 7)
496. In immunoprecipitation, how to elute proteins without IgG disturb? - (reply: 2)
497. Immonuprecipitation of membrane proteins, is it possible? - (reply: 1)
498. Non-specific binding of polyclonal antibodies - help (reply: 1)
499. Protein A- agarose or protein A- sepharose? - immunoprecipitation (reply: 1)
500. how to deal with protein degradation - (reply: 12)
501. Ratio of acrylamide to bis-acrylamide - different percentages for DNA and protein gels (reply: 1)
502. re-use Ni-NTA - (reply: 1)
503. WB vs ELISA ? - (reply: 2)
504. Question regarding muscle tissue homogenization - Why rotate homogenates after homogenization? (reply: 2)
505. HIs tag recognition problem - Ways to expose the HIS tag (reply: 1)
506. Prtocol for large scale protein expression - (reply: 1)
507. Protein precipitation compatible with Sucrose gradients? - What precipitation protocol wil function with a high % of sucrose? (reply: 5)
508. Methods needed for gene/protein identification and function - (reply: 2)
509. EMSA - What spacers do you have? (reply: 2)
510. Protein quantification - (reply: 3)
482. cytosolic and nucleus fractionation protocol plz - (reply: 2)
483. WB anti-caveoline1 not reproducible - (reply: 2)
484. inclusion bodies - (reply: 1)
485. How to correct the delay from detection to colletion in rpHPLC? - (reply: 8)
486. Native acrylamid gel - (reply: 10)
487. Western for membrane bound protein - (reply: 11)
488. Pierce ECL femto kit - loses effectiveness? (reply: 1)
489. Biotin EMSA Blocking buffer - Search for a suitable blocking buffer (reply: 1)
490. Help for sample preparation for 2-D - How can I get the protein for IEF (reply: 1)
491. who can help me with staining with 80kD to 130KD protein family? - it is very difficult for me to successfully staining with integrin (reply: 14)
492. Identification of monomers/dimers in whole cell lysate via SDS-PAGE - (reply: 3)
493. IPG strip burned - (reply: 2)
494. Too many problems in 2D - estimation? staining? running? (reply: 4)
495. ECL signal dies off rapidly - (reply: 7)
496. In immunoprecipitation, how to elute proteins without IgG disturb? - (reply: 2)
497. Immonuprecipitation of membrane proteins, is it possible? - (reply: 1)
498. Non-specific binding of polyclonal antibodies - help (reply: 1)
499. Protein A- agarose or protein A- sepharose? - immunoprecipitation (reply: 1)
500. how to deal with protein degradation - (reply: 12)
501. Ratio of acrylamide to bis-acrylamide - different percentages for DNA and protein gels (reply: 1)
502. re-use Ni-NTA - (reply: 1)
503. WB vs ELISA ? - (reply: 2)
504. Question regarding muscle tissue homogenization - Why rotate homogenates after homogenization? (reply: 2)
505. HIs tag recognition problem - Ways to expose the HIS tag (reply: 1)
506. Prtocol for large scale protein expression - (reply: 1)
507. Protein precipitation compatible with Sucrose gradients? - What precipitation protocol wil function with a high % of sucrose? (reply: 5)
508. Methods needed for gene/protein identification and function - (reply: 2)
509. EMSA - What spacers do you have? (reply: 2)
510. Protein quantification - (reply: 3)
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