2371. loss of His tag from protein made in mammalian cells? - Anyone have experience with His tag being cleaved off? (reply: 5)
2372. gst pull down assay - (reply: 2)
2373. GFP-protein signal vanish - (reply: 2)
2374. SDS-PAGE - new (reply: 4)
2375. overexpress his-tag protein, wrong size?! - (reply: 1)
2376. trouble in scaling-up protein expression - (reply: 5)
2377. viewing pentamers on a western blot - (reply: 3)
2378. Ni-NTA column purification - Ni-NTA column purification (reply: 1)
2379. How to measure labelled protein concentration? - (reply: 1)
2380. Histone like protein extraction from Gram positive - (reply: 1)
2381. His-tag protein purification - (reply: 1)
2382. HELP! selective scission - thrombin digestion (reply: 1)
2383. Glycerol effect on PAGE - (reply: 1)
2384. Removing Triton X-100 without denaturing the protein? - (reply: 6)
2385. help on western blots using plasma or serum - (reply: 1)
2386. NATIVE gel band - sample running at the side... - My NATIVE gel run produced a band which has hollowed out central with visible st (reply: 1)
2387. DTT or 2-mercaptoethanol - (reply: 1)
2388. why glycerol used in protein purification - (reply: 1)
2389. Same protein, different size on Western and IP ? - (reply: 2)
2390. 3.5 kDa peptide expression in periplasm or medium - (reply: 1)
2391. SDS-PAGE running with my sample widening? - my sample sort of grew fat in the separating gel...why? (reply: 8)
2392. SDS tracking dye running stragely - (reply: 2)
2393. How to extract Apoa1 from plasma - (reply: 3)
2394. How to use MG132? - (reply: 1)
2395. Cytochrome P450 expression - (reply: 11)
2396. how to interpret native gel result - (reply: 1)
2397. Storing PVDF membrane Help! - (reply: 4)
2398. question on complexes - protein-ligand complex detection in native gels (reply: 1)
2399. imidazole in protein purification - (reply: 2)
2400. Path length measuring protein concentration - (reply: 3)

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