1591. problem of protein expression - problem of protein expression (reply: 1)
1592. EMSA -- Chemiluminescent version by Pierce - (reply: 1)
1593. why some antibodies need overnight incubation for Western blot - (reply: 4)
1594. Percentage of SDS- PAGE gel for co-IP - (reply: 1)
1595. Protein extraction-what's in the pellet? - (reply: 6)
1596. Protein Transfection by Electroporation - (reply: 6)
1597. Lost: protein. Last seen prior to purification. - not present in flow through, washes or elutions! (reply: 2)
1598. Western blotting for ER - problems - (reply: 7)
1599. sds-page of hemocyte lysate proteins - (reply: 2)
1600. Coomassie G-250 vs R-250 - (reply: 3)
1601. 3T3 cells and lysis buffer recepie - western blot (reply: 1)
1602. silver staining trouble - (reply: 3)
1603. Precepitation during dialysis - (reply: 6)
1604. 2D Gel electrophoresis analysis software - Is a good one free available? (reply: 1)
1605. Storing PVDF membranes for N-terminal sequencing - Urgent! (reply: 1)
1606. gangliosides - (reply: 4)
1607. Does glycerol intefere an enzymatic reaction ? - (reply: 4)
1608. GST-tagged protein purification. - inclusion body formation of my GST taggged protein. (reply: 3)
1609. Question about the strategy of protein purification - (reply: 4)
1610. How to detect the protein concentration by UV light - (reply: 2)
1611. protein dialysis - (reply: 1)
1612. anti GFP - IP - (reply: 3)
1613. Immunoprecipitation-I can't see the heavy and light chain bands - (reply: 1)
1614. Why should add DTT and what kind protein assay I should use - (reply: 4)
1615. Problem with protein refolding - protein precipitate all the time !!! why (reply: 2)
1616. Is DTT better then B-mercapto - (reply: 8)
1617. One or two bands with different protein extraction buffer - (reply: 3)
1618. Western blotting signal problem - very weird and does not make any sense. (reply: 10)
1619. Coomassie Blue protein assay - Interference from phenolic acids (reply: 4)
1620. SDS-PAGE over heating and will western work from that gel? - sds page running and western blot of gel run under too much heating (reply: 9)

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