|
1531. Urea SDS-PAGE - why? (reply: 1)
1532. Urea IEF - urea for..? (reply: 1)
1533. Protein in active form - (reply: 2)
1534. which temperature should I run my 2. dimension gel at? - (reply: 3)
1535. Stacking Tris Molarity.. - how much.. same as resolving? (reply: 3)
1536. Increased signal in IP after peptide addition? - Immunoprecipitation signal amplification (reply: 2)
1537. Multiple bands on Western blot for an expressed protein - (reply: 7)
1538. who can compare adenoviral expression system and baculoviral expression system - (reply: 2)
1539. Inclusion body formation all or nothing? - (reply: 2)
1540. MBP fusion protein... - (reply: 6)
1541. TAPtagging in vivo - (reply: 4)
1542. far western - Would the expression of the protein in mammalian cells be enough for far wester (reply: 2)
1543. Protein formed ppt after stored at -80oC - (reply: 3)
1544. to check protein expression - (reply: 4)
1545. bubbles under vacuum-just curious - (reply: 2)
1546. Overexpression of Viral Protein in mammalian cell line - (reply: 3)
1547. GST-protein purification - help me please! (reply: 2)
1548. questions about native gel - (reply: 3)
1549. Is wetblotting better than semi-dry blotting for quantitative purpose? and why? - (reply: 1)
1550. are there any good system to express functional mammalian protein? - I tried using E.coli, but protein is not functional (reply: 15)
1551. question about "pa quality" - What's that? (reply: 3)
1552. Glycosylation - can I do anything with this? (reply: 3)
1553. AT1 receptor expression and quantification (WB) - need advice (reply: 1)
1554. quanitation of protein extracted with trizol - (reply: 7)
1555. Protein Sorting - The models of Protein sorting (reply: 1)
1556. Macrophages - (reply: 1)
1557. Non reducing Co-IP and 2 Ab that detect light chain only - Co-IP help! (reply: 4)
1558. unspecific binding of protein a sepharose - immunoprecipitation problem (reply: 1)
1559. PMSF in AChE purification :unsure: - (reply: 1)
1560. Immunoprecipitation - Can I boil then freeze? - (reply: 2)
1532. Urea IEF - urea for..? (reply: 1)
1533. Protein in active form - (reply: 2)
1534. which temperature should I run my 2. dimension gel at? - (reply: 3)
1535. Stacking Tris Molarity.. - how much.. same as resolving? (reply: 3)
1536. Increased signal in IP after peptide addition? - Immunoprecipitation signal amplification (reply: 2)
1537. Multiple bands on Western blot for an expressed protein - (reply: 7)
1538. who can compare adenoviral expression system and baculoviral expression system - (reply: 2)
1539. Inclusion body formation all or nothing? - (reply: 2)
1540. MBP fusion protein... - (reply: 6)
1541. TAPtagging in vivo - (reply: 4)
1542. far western - Would the expression of the protein in mammalian cells be enough for far wester (reply: 2)
1543. Protein formed ppt after stored at -80oC - (reply: 3)
1544. to check protein expression - (reply: 4)
1545. bubbles under vacuum-just curious - (reply: 2)
1546. Overexpression of Viral Protein in mammalian cell line - (reply: 3)
1547. GST-protein purification - help me please! (reply: 2)
1548. questions about native gel - (reply: 3)
1549. Is wetblotting better than semi-dry blotting for quantitative purpose? and why? - (reply: 1)
1550. are there any good system to express functional mammalian protein? - I tried using E.coli, but protein is not functional (reply: 15)
1551. question about "pa quality" - What's that? (reply: 3)
1552. Glycosylation - can I do anything with this? (reply: 3)
1553. AT1 receptor expression and quantification (WB) - need advice (reply: 1)
1554. quanitation of protein extracted with trizol - (reply: 7)
1555. Protein Sorting - The models of Protein sorting (reply: 1)
1556. Macrophages - (reply: 1)
1557. Non reducing Co-IP and 2 Ab that detect light chain only - Co-IP help! (reply: 4)
1558. unspecific binding of protein a sepharose - immunoprecipitation problem (reply: 1)
1559. PMSF in AChE purification :unsure: - (reply: 1)
1560. Immunoprecipitation - Can I boil then freeze? - (reply: 2)
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