1321. WB with cell culture medium - (reply: 2)
1322. precipitation of nuclear proteins during GST pull down assay - (reply: 1)
1323. Protein concentration measure with NanoDrop - (reply: 22)
1324. storage of membranes - (reply: 2)
1325. How long can RIPA buffer can be stored at 4C - (reply: 7)
1326. Protein extraction from soya meal using membrane - (reply: 1)
1327. Failure in Immunoprecipitation - No target protein found in eluate (reply: 4)
1328. Strange: mRNA expression goes up but protein goes down - (reply: 2)
1329. changing dialysis buffer - (reply: 7)
1330. GST purification multiple band problem - GST purification problem, multiple band (reply: 3)
1331. Immunoprecipitation of reduced proteins using Pierce kit - Immunoprecipitation using Pierce Kit (reply: 4)
1332. Signal Transduction - control for TGF beta signaling in HEk293 and VSMC (reply: 1)
1333. Purified Protein Aggregation/Multimerization Problem - (reply: 3)
1334. HELP! no expression in BL21 - (reply: 7)
1335. Protein analysis - (reply: 4)
1336. Phoshorylation in SDS Page - Visible shift? (reply: 4)
1337. Need help with WB/IP sensitivity issues - As in way too sensitive! (reply: 3)
1338. why not boiled before electrophoresis? - (reply: 9)
1339. Protein purification before mass-spec - need sample purification methods (reply: 4)
1340. streaking in 2D gels - (reply: 2)
1341. Memrane for Western Blot- which are the best? - (reply: 5)
1342. Anti-digoxigenin Immunoprecipitation of Proteins - (reply: 3)
1343. 1% SDS lysis buffer - (reply: 3)
1344. 2D PAGE problem - (reply: 7)
1345. question on pulldown - (reply: 6)
1346. reversible stain? - (reply: 4)
1347. what these components do in the buffer used in protein purification - (reply: 5)
1348. protein folding- looking for renaturation buffer - (reply: 1)
1349. temperature of silver staining - (reply: 3)
1350. ELISA Ab question - denatured protein and extract - If I extract denatured protein... (reply: 2)

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