1831. His-tagged proteins - bacteria or mammalian systems? - Which is better and why? (reply: 1)
1832. Please help me with bradford assays - (reply: 5)
1833. Cell line to be used for protein expression an purification - (reply: 8)
1834. Western Blot - membrane-bound protein boiling step - (reply: 1)
1835. Wet transfer vs Semi-dry transfer - (reply: 2)
1836. Storing gels . . . - (reply: 3)
1837. Protein expression problem - coexpression? (reply: 1)
1838. Protein Full Names - (reply: 3)
1839. Human IgA column - (reply: 1)
1840. Ponceau S as loading control on Westerns? - (reply: 3)
1841. protein samples on ice - how long is too long? (reply: 3)
1842. His tag protein purification problem - Binding of undesired protein (reply: 6)
1843. Colony lift followed by probing with a peptide; does this exist? - (reply: 6)
1844. protein diffusion in sds page gel - (reply: 2)
1845. Removal of lipids from protein samples - Supernatant protein extraction with acetone (reply: 1)
1846. Anion exchanger chromatography for virus purification - (reply: 1)
1847. Protein disappeared after TCA precipitation - (reply: 7)
1848. Streaks on 2D gels - (reply: 3)
1849. Plant protein extraction and purification - how to extract plant proteins and purify them? (reply: 1)
1850. what 's 7S and 11S subunit? - (reply: 3)
1851. Acrylamide stock for gels - which buffer (reply: 6)
1852. Help:Total protein extraction from soil - (reply: 2)
1853. SDS-PAGE band dissolution for scintillation counting - (reply: 5)
1854. low molecular weight proteins on SDS-PAGE - (reply: 1)
1855. PMSF or liquid nitrogen - (reply: 1)
1856. western blotting problem: b-actin is uneven - (reply: 6)
1857. SDS and transfer buffer......... - (reply: 1)
1858. 100A260/ml RIPA buffer - what does thi mean? (reply: 2)
1859. IP - How much protein needed? - (reply: 3)
1860. IP for few hours or ON - what's your favorite method? (reply: 10)

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