Immunoprecipitation - (Nov/23/2006 )
Apart from immunoprecipitation,is there any other method to isolate a protein from mammalian cell lysate?
-minnee-
QUOTE (minnee @ Nov 23 2006, 11:13 PM)
Apart from immunoprecipitation,is there any other method to isolate a protein from mammalian cell lysate?
Affinity chromatography, e.g. if the protein has a His-tag, you can do His-tag pull down
-dnafactory-
immunoprecipitations are a special case of pull-down assays; if you have a better affine protein than Ig to your protein of interest, try pull-down assays
-The Bearer-