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Immunoprecipitation - (Nov/23/2006 )

Apart from immunoprecipitation,is there any other method to isolate a protein from mammalian cell lysate?

-minnee-

QUOTE (minnee @ Nov 23 2006, 11:13 PM)
Apart from immunoprecipitation,is there any other method to isolate a protein from mammalian cell lysate?


Affinity chromatography, e.g. if the protein has a His-tag, you can do His-tag pull down

-dnafactory-

immunoprecipitations are a special case of pull-down assays; if you have a better affine protein than Ig to your protein of interest, try pull-down assays

-The Bearer-