Blotting for weekend rather than overnight - What problems could this cause? Will it work? (Nov/30/2006 )

I'm doing a western blot. After the transfer stage, I usually block the membrane (PVDF) in casein solution overnight. Unfortunately, I won't be here tomorrow - if I block it in the fridge till Monday (so 4 nights instead of 1 night), will this be a major problem or is there no such thing as 'too much blocking'?

---

not mainly to the blot but you have to asure that the blocking solution will not be contaminated by fungi or bacteria, and that it permanently rocks; you may dilute your blocking solution to the half

---

Thanks Kosmodrom, that's great. I might pop a small amount of azide in to preserve it.

Cheers,

Chris

---