what speed to centrifuge down agarose bead? - (Nov/28/2006 )

I saw some protocals said at low speed to avoid crashing the bead, some just at full speed, some pulse at full speed (ie 5 sec at full speed). I did at low speed ,feel the bead is not packed enough, so it's hard for me to aspirate the sup.

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I spin the amersham one at 12000g 30s. It's OK
I did the same with beads from an other provider, but it was bad for them. they were compacting.
use the protocol given by the provider.

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I agree with missele - if there is no protocol of the manufacturer be defensive and try to restrict rpm to the lowest but efficient speed;

beside prevention of the breaking of beads, I do not know if a rapid pelleting may have effects on the specific binding, I mean if a high g-number would force to part some of the binding partners...

any suggestions to this point??

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using M2 agarose beads from sigma, 2000rpm in a swing out rotor for 30'' do the job.

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centrifuge at very low speed (2000) and if its impossible to remove super increase very gradually until you are able to. (pray not to crush the beads!)

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