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vortexing of antibodies upon dilution in 3% skim milk - (Jan/24/2007 )

dear all
I am a beginner in western blot. is it possible to vortex antibodies (primery &secondary) upon dilution in 3% skim milk ? or is it better to mix by pipetting up and down?

-raneem-

I just mix them by inverting. Vortexing is not neccessary and may harm them.

-biomaus-

I vortex antibody sol. in milk, gently and briefly for 1-2 seconds.

-scolix-

QUOTE (scolix @ Jan 24 2007, 05:40 PM)
I vortex antibody sol. in milk, gently and briefly for 1-2 seconds.

I do the same as scolix

-Pumuki-