sepharose bead - (Jan/19/2007 )

If pack a sepharose bead into a column, can that only be used for purification by gravity?
Thanks!

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What are you trying to do with it? you can run it with pump.

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if it is certified up to which pressure beads are stable you can use it with pressure e.g. HPLC

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pump is a kind of pressure, isn't it? It's not flow by gravity, right?

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It provides a constant flow rate. The pressure is proportional to the flow rate you apply.

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both yes.

If you have a large volume (say 200ml) of sample to load into the sepharose bead column, it is better to use a pump or else you will be staring at the column and adding sample again and again.

If you have a small volume (say <30ml), it is OK to use gravity.

You can use a pump with a flow rate of 1ml/min.

Hope this help.

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