Which water is better for chemical preparation ? - water grade for protein purification (Jan/17/2007 )

Hi, all.

I 'm working on protein purification by using ion-exchange chromatography and His-trap columns.
I have some question

1. Between tripple distilled water and type I water from water purification system, PURELAB ultra (bioscience grade), which is the better for my works ?

2. Would PURELAB ultra water is good enough for my works ?

This is diagram of bioscience water flow http://www.elgalabwater.com/files/Document/Ultra Bioscience Flow Diagram.jpg://http://www.elgalabwater.com/files/D...low Diagram.jpg://http://www.elgalabwater.com/files/D...low Diagram.jpg://http://www.elgalabwater.com/files/D...low Diagram.jpg

-Kidd-

QUOTE (Kidd @ Jan 18 2007, 03:05 AM)

some if not most proteins need beside a certain ionic strength some trace elements to build stable confirmations which means ultra pure water is not necessary for the proteins;

on the other hand, if columns are re-used it will be better to use ultra pure water

-The Bearer-

i use purelab ultra in my lab for years now, and that's really appropriate.
I've no financial interest on them

-fred_33-