Resolve High Molecular Weight Proteins in SDS-PAGE - (Feb/07/2007 )
I met the problems on resolve the high molecular weight proteins from range 250KD to 500KD. 6% SDS-PAGE can not resolve it. However, 4% SDS-PAGE will cause smear and cann't resolve clearly.
Anyone have experience on the 4% SDS-PAGE gel? 
-Laurence Lao-
QUOTE (Laurence Lao @ Feb 8 2007, 04:20 AM)
I met the problems on resolve the high molecular weight proteins from range 250KD to 500KD. 6% SDS-PAGE can not resolve it. However, 4% SDS-PAGE will cause smear and cann't resolve clearly.
Anyone have experience on the 4% SDS-PAGE gel?
Anyone have experience on the 4% SDS-PAGE gel?
Perhaps you could pour a gradient gel of low percentage to resolve the higher molecular weight proteins.
-Mountainman-
I've heard about adding glycerol in the gel, for high molecular weight.
unfortunately I have no experience with that.
I'll try to find the guy who told me about his glycerol gels migrating for 36 hours, many years ago. he was separating myosin isoforms.
-Missele-
QUOTE (Laurence Lao @ Feb 8 2007, 08:20 AM)
I met the problems on resolve the high molecular weight proteins from range 250KD to 500KD. 6% SDS-PAGE can not resolve it. However, 4% SDS-PAGE will cause smear and cann't resolve clearly.
Anyone have experience on the 4% SDS-PAGE gel?
Anyone have experience on the 4% SDS-PAGE gel?
do a gradient gel ranging from 2-4% to 14-16%; it also depends on if you like to separate well medium sized polypeptides (~20-100 kDa)
-The Bearer-