Protein high molecular weight standards for gel filtration - (Feb/13/2007 )

I am keen to get together some high molecular weight standards between 800kDa and 2 million Da. I can get hold of blue dextran but what other proteins are commonly used, relatively cheap and suitable in gel filtration as weight standards.

Commonly used protein SEC calibration are:

Feritine 440000 ( 1mg\ml ) should be used
Aldolase 160000 ( up to 5 mg\ml)
Catalase 240000 up to 5 mg\ml)
Albumin 67000 up to 5 mg\ml)
Ovalbumin 42000 up to 5 mg\ml)
Ribonuclease A 12500 up to 5 mg\ml)
Chymotrypsinogen 24000 up to 5 mg\ml)


Attention: Blue Dextran must be used fresh and this protein have some absorption on resin . Use this protein at 2mg\ml only to check dead volume of your column. BSA often may fall out from your calibration curve because of its abnormal behavior. After calibration of your SEC column don't forget probe volume that you use for calibration. Typical velocity for column diam 26mm - 18ml\hour, 16 mm column - 12ml\hour