His tag purification - competition with imidazole or histidine? (Mar/16/2007 )

Does anyone have experience with either imidazole or histidine in his tag purification?
Thinking of using a Invitrogen column, and nickel and then either imidazole or histidine..
Thanks!

-lenenoerby-

well imidazole seems more used to me.

-fred_33-

QUOTE (lenenoerby @ Mar 16 2007, 10:57 AM)

Does anyone have experience with either imidazole or histidine in his tag purification?
Thinking of using a Invitrogen column, and nickel and then either imidazole or histidine..
Thanks!

Thanks a lot - I'll order imidazole..

-lenenoerby-

QUOTE (lenenoerby @ Mar 16 2007, 04:47 AM)

QUOTE (lenenoerby @ Mar 16 2007, 10:57 AM)

Does anyone have experience with either imidazole or histidine in his tag purification?
Thinking of using a Invitrogen column, and nickel and then either imidazole or histidine..
Thanks!

Thanks a lot - I'll order imidazole..

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-circlepoint-

imidazole for native protein isnt it?

-timjim-

mostly imidazole. Ofcourse, you will have to optimise the imidazole conc.

-scolix-