How to purify protein secreted in medium - (Mar/07/2007 )

I am expressing the protein as asecretory protein. My protein after comes in medium . please tell how to purify it.

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what about inmunoprecipitating it?

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try ammonium sulphate precipitation, subsequently followed by dialysis;

if you need higher enrichment or purity, think of biochromatographical steps after ammonium sulphate precipitation f.i. hydrophobic interaction chromatography or affinity chromatography

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