can u guess the molecular weight of a raised antibody? - (Mar/13/2007 )
well,i got a strech of peptide synthesized and antibody raised from it.is it a polyclonal antibody because in western blots it picks up many bands. can i ever know the molecular weight of this antibody. In general what is the molecular weight of an antibody.
-min04-
The western shows you the MW of the protein that the antibody recognizes, not the MW of the antibody itself. I think the MW of antibodies are different depending on what type of Ig molecules they are made of, IgA, IgG, etc. I don't know if a table exists anywhere...
-WAstate-
QUOTE (WAstate @ Mar 13 2007, 05:11 PM)
The western shows you the MW of the protein that the antibody recognizes, not the MW of the antibody itself. I think the MW of antibodies are different depending on what type of Ig molecules they are made of, IgA, IgG, etc. I don't know if a table exists anywhere...
IgG heavy chain ~55 kDa, light chain ~25 kDa in SDS PAGE
-The Bearer-
QUOTE (WAstate @ Mar 13 2007, 08:11 AM)
The western shows you the MW of the protein that the antibody recognizes, not the MW of the antibody itself. I think the MW of antibodies are different depending on what type of Ig molecules they are made of, IgA, IgG, etc. I don't know if a table exists anywhere...
For IgG between 150-190 kDA depend on glycosylation ( In sample buffer without bME on SDSPAAG ) with bME as The Bearer say
-circlepoint-