2D Electrophoresis for protein inclusion bodies/lysate? - sample preparation? (Jun/26/2008 )

hia!
does any1 have a handy protocol for sample preparation for 2D Electrophoresis for protein inclusion bodies/lysate?
i have a high protein load for the protein over expressed but very little of host protein r seen.. so i get a blotch at the position of my protein. and its surrounding... data difficutl to interpret.

also i get a lot of streaking/dragging in my gel.. how do i avoid that?

i preferably wish to avoid any protein loss / precipitaion in the sample preparation.. is there a way? thanks!

Please check it out at http://keck.med.yale.edu/pdfs/Ettan%20DIGE...er%20Manual.pdf