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Protein extraction from periplasmic space - a simple protocol anyone? (Jul/02/2008 )

I was just wondering if anyone knows a simple method for the extraction of periplasmic protein from e.coli without too much contamination by cytoplasmic proteins?

-khommy-

QUOTE (khommy @ Jul 2 2008, 11:12 PM)
I was just wondering if anyone knows a simple method for the extraction of periplasmic protein from e.coli without too much contamination by cytoplasmic proteins?

Check these links; let us know what finally worked!
http://search.vadlo.com/b/q?sn=158621799&a...tein+&rel=0
..

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