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to explain why i see two forms the same protein in SDS-PAGE - (Jun/25/2008 )

I need to explain why they appear two bands of the same protein dimero and the monomer when rune a SDS-PAGE using BME 5% like reducing agent in sample buffer
Thanks for your help

-giardita-

Try a bit more BME, and try boiling the samples for 5-10 minutes before you load.

-swanny-