Protease Cocktail Inhibitor Complete from Roche? - (Jun/02/2007 )
Anyone that ever used these tablets? I am very surprised that they do not specify what is the final concentration in the final solution, they just say you should dissolve one tablet in 2 ml of redistilled water adn that it is enough for 50ml sample. Is that all?? Kind of very unspecific when you want to have more detailed information as to how much to use in your experiment.
Do you know if this cocktail causes bacterial cell lysis?
-smoochiepie79-
QUOTE (smoochiepie79 @ Jun 2 2007, 04:57 PM)
Anyone that ever used these tablets? I am very surprised that they do not specify what is the final concentration in the final solution, they just say you should dissolve one tablet in 2 ml of redistilled water adn that it is enough for 50ml sample. Is that all?? Kind of very unspecific when you want to have more detailed information as to how much to use in your experiment.
Do you know if this cocktail causes bacterial cell lysis?
Do you know if this cocktail causes bacterial cell lysis?
we have use it routinely for several years (formerly Boehringher Mannheim product); as we have no problems with proteolysis, we think it works; it is true, they do not specify the ingredients but they detail which proteases are blocked to what extent; at the moment they offer free trial packs...
-The Bearer-
we also use that tablets and I agree with Bearer.
-Pumuki-
QUOTE (The Bearer @ Jun 2 2007, 10:27 PM)
QUOTE (smoochiepie79 @ Jun 2 2007, 04:57 PM)
Anyone that ever used these tablets? I am very surprised that they do not specify what is the final concentration in the final solution, they just say you should dissolve one tablet in 2 ml of redistilled water adn that it is enough for 50ml sample. Is that all?? Kind of very unspecific when you want to have more detailed information as to how much to use in your experiment.
Do you know if this cocktail causes bacterial cell lysis?
Do you know if this cocktail causes bacterial cell lysis?
we have use it routinely for several years (formerly Boehringher Mannheim product); as we have no problems with proteolysis, we think it works; it is true, they do not specify the ingredients but they detail which proteases are blocked to what extent; at the moment they offer free trial packs...
Well, here we are using the inhibitors as to test something different than their usual function is but anyway, thanks guys:)
-smoochiepie79-
You can ask Roche specially for your application or any doubt.
-Pumuki-