native PAGE - (Jul/16/2007 )

am not able to visualize bands in native page and rather the protein is not moving down the gel can anyone tell me the probable solution to it....

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may be the wrong buffer system (pH) which does not fit to the pI´s of your proteins was choosen; native PAGE is tricky and need some trials if there is no established protocol

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hi
first of all you need to find out PI of your protein then accordingly you choose the ph of resolving gel and of running buffer.

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