isolation of protein complex by use percoll gradient - (Jul/16/2007 )
hi all,
my boss had asked me to isolate a protein complex from cell lysate by using percoll gradient or sucrose gradient. but we have a problem that the elution should be so diluted.
anyone have method to concentrate the elution without distrupt the protein complex
thx!!!!!!!!!!!!!!!!!!!
-balllai-
QUOTE (balllai @ Jul 16 2007, 12:19 PM)
hi all,
my boss had asked me to isolate a protein complex from cell lysate by using percoll gradient or sucrose gradient. but we have a problem that the elution should be so diluted.
anyone have method to concentrate the elution without distrupt the protein complex
thx!!!!!!!!!!!!!!!!!!!
my boss had asked me to isolate a protein complex from cell lysate by using percoll gradient or sucrose gradient. but we have a problem that the elution should be so diluted.
anyone have method to concentrate the elution without distrupt the protein complex
thx!!!!!!!!!!!!!!!!!!!
membrane filtration (various companies f.i. Pierce) or speed vac concentration (lyophilization)
-The Bearer-