transfer buffer question - anyone ever use TBS as a transfer buffer? (Jul/18/2007 )
Hi All!
Has anyone ever heard of using plain old TBS as a transfer buffer when using nitrocellulose ( or PVDF, for that matter) ? I haven't heard of this , but some colleagues were arguing over this and I thought I'd throw it out to the bioforum crew for any potential input.
LONG LIVE TOWBIN!
monkeybaji
-monkeybaji-
hello,
I always put 20% MeOH, EtOH or iPrOH in the buffer for transfer to nitrocellulose
Seb
-tryptofan-
there may exist a confusion between TBS and TGS...
but why not ? i don't have used it, as pH is different and buffering capacity change...
Moreover there is more salt in TBS.
-fred_33-
QUOTE (monkeybaji @ Jul 18 2007, 07:41 PM)
Hi All!
Has anyone ever heard of using plain old TBS as a transfer buffer when using nitrocellulose ( or PVDF, for that matter) ? I haven't heard of this , but some colleagues were arguing over this and I thought I'd throw it out to the bioforum crew for any potential input.
LONG LIVE TOWBIN!
monkeybaji
Has anyone ever heard of using plain old TBS as a transfer buffer when using nitrocellulose ( or PVDF, for that matter) ? I haven't heard of this , but some colleagues were arguing over this and I thought I'd throw it out to the bioforum crew for any potential input.
LONG LIVE TOWBIN!
monkeybaji
too much NaCl; Towbin protocol uses Tris, glycine, SDS and methanol, doesn´t it?
-The Bearer-
yes,
i do use TBS as transfer buffer for mt westerns with 0.1%sds and 4% methanol for NC membrane. and i have had fantastic results
QUOTE (monkeybaji @ Jul 18 2007, 10:41 AM)
Hi All!
Has anyone ever heard of using plain old TBS as a transfer buffer when using nitrocellulose ( or PVDF, for that matter) ? I haven't heard of this , but some colleagues were arguing over this and I thought I'd throw it out to the bioforum crew for any potential input.
LONG LIVE TOWBIN!
monkeybaji
Has anyone ever heard of using plain old TBS as a transfer buffer when using nitrocellulose ( or PVDF, for that matter) ? I haven't heard of this , but some colleagues were arguing over this and I thought I'd throw it out to the bioforum crew for any potential input.
LONG LIVE TOWBIN!
monkeybaji
-nirav-