Limit of NaCl in the sample - (Jul/19/2007 )

Hi
I have in my samples 500mM of NaCl. hope this is not a problem for the migration of it
someone knoes the limit of NaCl we can have in a sample?

-DoD-

is it proteins ? DNA ? RNA?
i think it's quite hard...
i've run succesfully nuclear proteins containing 300mM KCl and 1M KCL
NaCl not used with proteins.

-fred_33-

QUOTE (DoD @ Jul 19 2007, 03:50 PM)

Hi
I have in my samples 500mM of NaCl. hope this is not a problem for the migration of it
someone knoes the limit of NaCl we can have in a sample?

it is not a matter of conc but total amount;

for SDS PAGE of a normal 10-20 µl sample, not more than 50 mM NaCl

-The Bearer-

QUOTE (The Bearer @ Jul 19 2007, 11:33 AM)

QUOTE (DoD @ Jul 19 2007, 03:50 PM)

Hi
I have in my samples 500mM of NaCl. hope this is not a problem for the migration of it
someone knoes the limit of NaCl we can have in a sample?

it is not a matter of conc but total amount;

for SDS PAGE of a normal 10-20 µl sample, not more than 50 mM NaCl

It's for a SDS page and the buffer of my sample is with 500mM NaCl :-(. I will load 30µl sample.
So You think that i will have some problem with this concentration of NaCL?

-DoD-

QUOTE (DoD @ Jul 20 2007, 07:45 AM)

QUOTE (The Bearer @ Jul 19 2007, 11:33 AM)

QUOTE (DoD @ Jul 19 2007, 03:50 PM)

Hi
I have in my samples 500mM of NaCl. hope this is not a problem for the migration of it
someone knoes the limit of NaCl we can have in a sample?

it is not a matter of conc but total amount;

for SDS PAGE of a normal 10-20 µl sample, not more than 50 mM NaCl

It's for a SDS page and the buffer of my sample is with 500mM NaCl :-(. I will load 30µl sample.
So You think that i will have some problem with this concentration of NaCL?

definitely! do not do it! desalt by precipitation f.i. Wessel-Flügge protocol (methanol-chloroform protocol)

-The Bearer-

you may do a dialysis if you have enough material.

conplement to my reply : i have loaded 5 µl only

-fred_33-

QUOTE (fred_33 @ Jul 20 2007, 04:00 AM)

you may do a dialysis if you have enough material.

conplement to my reply : i have loaded 5 µl only

I will be good for this purpose to apply microdialyse system prepared from eppendorf cap

-circlepoint-