Limit of NaCl in the sample - (Jul/19/2007 )

Hi
I have in my samples 500mM of NaCl. hope this is not a problem for the migration of it
someone knoes the limit of NaCl we can have in a sample?

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is it proteins ? DNA ? RNA?
i think it's quite hard...
i've run succesfully nuclear proteins containing 300mM KCl and 1M KCL
NaCl not used with proteins.

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it is not a matter of conc but total amount;

for SDS PAGE of a normal 10-20 µl sample, not more than 50 mM NaCl

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It's for a SDS page and the buffer of my sample is with 500mM NaCl :-(. I will load 30µl sample.
So You think that i will have some problem with this concentration of NaCL?

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definitely! do not do it! desalt by precipitation f.i. Wessel-Flügge protocol (methanol-chloroform protocol)

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you may do a dialysis if you have enough material.

conplement to my reply : i have loaded 5 µl only

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I will be good for this purpose to apply microdialyse system prepared from eppendorf cap

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