IPG strip burned - (Nov/24/2007 )
Hi!
After active rehydration, isoelectric focusing by placing a wet wick under both ends of the IPG strips (AMERSHAM), one of my 12 strips burned. I used a BIORAD PROTEAN IEF for first dimension...
Now I have a slightly 1cm-long burned lane near the anode!
Can I still use the 24cm tray (about 900€) for IEF???? OR shall I buy a new one? Is there might be a thermic problem? OR some loss of IEF capacity?
Has anybody else some experience with regard to this topic?
Thanks for help!
-caine1203-
QUOTE (caine1203 @ Nov 24 2007, 11:43 AM)
Hi!
After active rehydration, isoelectric focusing by placing a wet wick under both ends of the IPG strips (AMERSHAM), one of my 12 strips burned. I used a BIORAD PROTEAN IEF for first dimension...
Now I have a slightly 1cm-long burned lane near the anode!
Can I still use the 24cm tray (about 900€) for IEF???? OR shall I buy a new one? Is there might be a thermic problem? OR some loss of IEF capacity?
Has anybody else some experience with regard to this topic?
Thanks for help!
After active rehydration, isoelectric focusing by placing a wet wick under both ends of the IPG strips (AMERSHAM), one of my 12 strips burned. I used a BIORAD PROTEAN IEF for first dimension...
Now I have a slightly 1cm-long burned lane near the anode!
Can I still use the 24cm tray (about 900€) for IEF???? OR shall I buy a new one? Is there might be a thermic problem? OR some loss of IEF capacity?
Has anybody else some experience with regard to this topic?
Thanks for help!
IEF runs with a constant increase of voltage, depending on your power supply, in steps or as a ramp; may be the increase was too steep;
if your IEF module is still usable should be checked by a competent technician
-The Bearer-
QUOTE (caine1203 @ Nov 24 2007, 07:43 PM)
Hi!
After active rehydration, isoelectric focusing by placing a wet wick under both ends of the IPG strips (AMERSHAM), one of my 12 strips burned. I used a BIORAD PROTEAN IEF for first dimension...
Now I have a slightly 1cm-long burned lane near the anode!
Can I still use the 24cm tray (about 900€) for IEF???? OR shall I buy a new one? Is there might be a thermic problem? OR some loss of IEF capacity?
Has anybody else some experience with regard to this topic?
Thanks for help!
After active rehydration, isoelectric focusing by placing a wet wick under both ends of the IPG strips (AMERSHAM), one of my 12 strips burned. I used a BIORAD PROTEAN IEF for first dimension...
Now I have a slightly 1cm-long burned lane near the anode!
Can I still use the 24cm tray (about 900€) for IEF???? OR shall I buy a new one? Is there might be a thermic problem? OR some loss of IEF capacity?
Has anybody else some experience with regard to this topic?
Thanks for help!
Has anyone else a burned IEF module????????
-caine1203-