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Wet transfer vs Semi-dry transfer - (Jun/03/2006 )

Which transfer system (wet or semi-dry) does your lab prefer use and why? What are the advantages/disadvantages of each system? ***Thanks your responses are greatly appreciated***

-sv112-

my lab uses a semi-dry transfer unit.

we like it because our transfers are faster and require a lot less buffer.

also, we use very large gels (non-standard) and we can fit them on the unit we purchased.

-mdfenko-

we use the semi dry transfert apparatus for reasons that mdfenko mentionned. But it allows also very good protein transfert overnight at 5V which wet transfert doesn't allow in our hands.

-fred_33-