Buffer storage question - (Oct/08/2008 )

Hi,

I am hoping to try out a new protocol for western blots and for one of the steps, the protocol says to dilute my protein samples in a buffer before loading onto polyacrylimide gel.
The buffer in question contains
-125mM Tris, pH6.8
-2% SDS
-10% Glycerol
-0.2% Bromophenol Blue
-5mM DTT

I am wondering if anyone knows if it would be possible to make up a stock solution of this buffer and freeze/refrigerate aliquots? Or would I have to make the buffer up fresh each time I need the buffer?

Thanks

Hi,

I am hoping to try out a new protocol for western blots and for one of the steps, the protocol says to dilute my protein samples in a buffer before loading onto polyacrylimide gel.
The buffer in question contains
-125mM Tris, pH6.8
-2% SDS
-10% Glycerol
-0.2% Bromophenol Blue
-5mM DTT

I am wondering if anyone knows if it would be possible to make up a stock solution of this buffer and freeze/refrigerate aliquots? Or would I have to make the buffer up fresh each time I need the buffer?

Thanks

QUOTE (clarem @ Oct 8 2008, 02:46 AM)

Hi,

I am hoping to try out a new protocol for western blots and for one of the steps, the protocol says to dilute my protein samples in a buffer before loading onto polyacrylimide gel.
The buffer in question contains
-125mM Tris, pH6.8
-2% SDS
-10% Glycerol
-0.2% Bromophenol Blue
-5mM DTT

I am wondering if anyone knows if it would be possible to make up a stock solution of this buffer and freeze/refrigerate aliquots? Or would I have to make the buffer up fresh each time I need the buffer?

Thanks

you should freeze as DTT hydrolyzes within hours; all other ingredients are stable w/o freezing