Separation of piperidine from proteins after AIC - (Dec/14/2007 )
I have many fractions from protein anion exchange chromatography purification. Fractions contain (in addition to proteins) buffer with piperidine and 0-1M NaCl, pH = 11.
Could you suggest, how can I easily and effectively remove proteins (from many fractions) from that piperidine buffer?
I would like to use acetone to precipite proteins (because it is cheap, effective and proven), but I do not know whether the piperidine would not interfere somehow with the protein acetone precipitation. Do you know it?
Many thanks.
victor
-victor.m-
acetone or ammonium sulfate precipitation should work or you could try diafiltration with an amicon stirred cell.
-mdfenko-