4741. Problems cloning cDNA using Pfx - Problem (reply: 2)
4742. Degenerate primers - (reply: 1)
4743. Single cell gel electrophoresis (comet assay) - (reply: 1)
4744. gene deletion via the RED recombinase method - are you experienced? (reply: 3)
4745. Transforming DH5 alpha cells with pUC19 vector - Cloning (reply: 3)
4746. DNA pellet (?) floats in CTAB precipitation - (reply: 2)
4747. searching for practical protocols - (reply: 1)
4748. pSUP5011 - what is the molecular size of the plasmid pSUP5011 (reply: 1)
4749. Tissue storage for RNA isolation - (reply: 4)
4750. primer optimization - (reply: 1)
4751. problem with casting PAG - large number of persistent air bubbles in a gel (reply: 7)
4752. degraded DNA - (reply: 1)
4753. plasmid extraction and antibiotics matters... - (reply: 4)
4754. PCR on plasmids? - (reply: 2)
4755. Plasmid methylation problem - Plasmid methylation (reply: 3)
4756. DNase treatment for removing DNA from RNA samples - (reply: 1)
4757. difficulty in cloning oligos into vector - cloning difficulties (reply: 1)
4758. PCR with T7 promoter/T7 terminator - (reply: 5)
4759. Pfx PCR problems - (reply: 1)
4760. Self ligation of vector - (reply: 2)
4761. primers for Q-PCR - (reply: 2)
4762. Topo cloning no colonies - (reply: 3)
4763. Purification of Genomic DNA - (reply: 2)
4764. Storage of RT-PCR products - (reply: 2)
4765. Store ligation reaction for repeat transformation? - (reply: 3)
4766. Transformation of an unstable gene - (reply: 1)
4767. Detecting 12 nt differences in a 600+ bp pcr product - (reply: 2)
4768. oligo annealing - how many oligos for annealing (reply: 1)
4769. In situ Hybridization problem with TCS solution?? - (reply: 1)
4770. Tool for searching optimal oligonucleotide, ISH? - (reply: 2)

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