1081. pcr issue-gene not amping - (reply: 3)
1082. Forward n reversre primer Tm difference is 10 degree - (reply: 1)
1083. Primer mismatch bother me! - when enzyme sites added at 5' ends, mismatch increases. (reply: 1)
1084. Question about maxi plasmid preparation - (reply: 1)
1085. Promoter studies - (reply: 3)
1086. Smearing in PCR - (reply: 4)
1087. Troubleshooting RT-PCR - (reply: 7)
1088. Why using heated-top or add mineral oil in PCR - (reply: 2)
1089. Ligation, plasmid rescue problem! - PLasmid transformation after ligation problem (reply: 7)
1090. do I get right PCR product - (reply: 20)
1091. Why anneal at Tm? - (reply: 2)
1092. How to determine DNA sequence is from male or female? - (reply: 5)
1093. EMSA problem - (reply: 4)
1094. yeast colony PCR - How to improve yeast colony PCR (reply: 7)
1095. BLOOD storage and PCR problems! HELP - (reply: 1)
1096. Anyway to lyse bacteria but do not lyse WBC? - in human blood samples (reply: 2)
1097. fibroblast marker genes - fibroblast marker genes (reply: 1)
1098. stop-transfer mechanism - (reply: 1)
1099. EGFP constructed in T-vector can express green fluorescence? - SOS (reply: 6)
1100. Sprinting fragments? - (reply: 1)
1101. pcr problems - pcr to solve (reply: 2)
1102. Calculating protein size - how to calculate the protein size when cloned in pet vector (reply: 1)
1103. Thermostable Reverse Transcriptase for RT-PCR - (reply: 3)
1104. figure out the size of amplicon using known primer - (reply: 3)
1105. Blunt end cloning question - (reply: 1)
1106. Molecular Cloning: A Laboratory Manual (3-Volume Set) - buy now or later? (reply: 4)
1107. Primer concentration... - (reply: 5)
1108. Separation of DNA bands of sizes 8.86 kb and 9.5 kb - (reply: 3)
1109. Strange background in lanes using Pierce Lightshift EMSA - (reply: 1)
1110. from Trizol to phenol/chloroform for DNA extraction - (reply: 1)

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