Tissue storage for RNA isolation - (May/22/2005 )

Please, give your thought! Thanks in advance.

When we first do an isolation then we first have to do a mini prep to test for the quality before we do a maxi or a bigger prep of a sample. MY question is i have to do total RNA isolation from a tumour tissue how can i just isolate a little RNA from this tissue and then freeze the rest of the tissue for later use? Would not this Freeze-thaw circle damage the tissue for later use?

Thanks.

well, does this mean that noone has ever isolated total RNA from a tumour tissue before? Would the thaw and freeze circle affect the quality of it for later use?


Hope very much for an idea!


Thanks again!

RNA in tissue cannot stand repeat freeze-thaw cycles. You can store tissue in a solution called RNAlater (from Qiagen and Ambion) or divide your tissue into small pieces and store them in liquid nitrogen.

If you are sure your method works, you don't need to do 'miniprep' for every sample.