1651. Help with Ligation - (reply: 3)
1652. Apyrase in pyrosequencing - (reply: 1)
1653. Bacterial housekeeping genes - What genes are commonly used as housekeeping controls in expression st (reply: 1)
1654. Problem with Ligation - (reply: 4)
1655. do you subtract gel base intensity from PCR band intensity? - urgent require (reply: 3)
1656. HIV-2 and MTT assay, cells lines to use - (reply: 2)
1657. How isolate a 4kbp gene?! - (reply: 3)
1658. unable to digest my recombinant plasmid! - (reply: 9)
1659. Can bacteria grow without a plasmid or am i stupid? - or did ampicillin wear out? (reply: 5)
1660. 2kb PCR - Help!!!!!!!!!! (reply: 9)
1661. what is the maximum DNA I can load - Southern blot problem (reply: 3)
1662. Urgent amp agar plate question! - Help with stupid question (reply: 22)
1663. pcr product degradation - (reply: 1)
1664. formamide and DNA denaturation - (reply: 1)
1665. DH5a genotype - phi80delM15 (constitutive or regulated)? - (reply: 1)
1666. EMSA witj antibodies against TFII factors - (reply: 3)
1667. RNA contamination? - (reply: 4)
1668. RNA purification from pancreas - troubles with the pancreas (reply: 1)
1669. How to dilute loading dye? (For gel electrophoresis) - (reply: 6)
1670. Agarose 'rafts' blocking the top of sample well - Difficulty pouring a 3% gel (reply: 4)
1671. Phenol-Chloroform at pH 8: Where's the RNA go? - (reply: 2)
1672. RNA extraction from plant tissue - all method are welcome (reply: 7)
1673. E. coli transformation issues - old plasmids (reply: 7)
1674. Degenerate Primers and dI - (reply: 5)
1675. Which UTR to use after T7 promoter? - (reply: 1)
1676. Need to get 3' end sequence! - 3' end sequencing (reply: 4)
1677. titre - (reply: 1)
1678. RNA contamination: how to get ride off it from extracted DNA? - (reply: 2)
1679. Eclone = ? for In Situ and mRNA injection - What is the official gene name for "eclone" ? (reply: 1)
1680. designing a primer - (reply: 5)

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