2431. Is multiple freezing-thawing harmful to cDNA? - (reply: 3)
2432. 2 PCR product to be inserted into 1 expression vector - (reply: 2)
2433. Need a FRET PCR protocol - (reply: 1)
2434. RACE problems - 5-terminal amplication (reply: 1)
2435. for the PCR primer what is better the hairpin or the self annealing? - (reply: 9)
2436. transcription in prokaryotes. - is whole shine-dalgarno sequence required? (reply: 1)
2437. Uncut genomic DNA - Migrate through the gel (reply: 3)
2438. Uncut plasmid DNA - (reply: 6)
2439. Using Picogreen to quantitate DNA - Questions about how to make this work! (reply: 2)
2440. analysis of DNA using BamH1 and agarose gel electrophoresis - interpretation of results! (reply: 5)
2441. DNA isolation kits - (reply: 4)
2442. What do you mean by other letters aside from A, C, G, and T in DNA sequencing r - (reply: 3)
2443. RT-PCR Troubles.. - (reply: 3)
2444. Bad sequences - (reply: 6)
2445. Plasmid DNA Isolation problems, contaminating chromosoma DNA - How to isolate large inserts by miniprep and without chromosomal smearing (reply: 4)
2446. Ligation problems - (reply: 12)
2447. mobility of protein band over PAGE - please suggest (reply: 1)
2448. How can I dissolve 6%polyacrylamide gel? - (reply: 2)
2449. Isolation of plasmid DNA - alkaline lysis method (reply: 3)
2450. expression problems in mammalian cells - (reply: 2)
2451. Isolation of plasmid DNA - chemicals used-why?? (reply: 5)
2452. genomic DNA contamination in plasmid DNA prep - (reply: 1)
2453. so i have reached a dead end. - (reply: 4)
2454. 20% chelex extraction method for forensic samples - (reply: 1)
2455. Is this kind of primer OK? - (reply: 2)
2456. BAC recombineering - BAC elctroporation (reply: 4)
2457. real time PCR-Primer question - (reply: 1)
2458. RNA extraction of a very, very small tissue sample - (reply: 2)
2459. RNA extraction methods for callus - (reply: 1)
2460. EMSA problems - (reply: 2)

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