5071. Lentiviral vector for infecting primary cells? - Any suggestion for a suitable vector? (reply: 1)
5072. BclI working efficiency - enzyme cutting (reply: 5)
5073. In Gel Ligation - Anyone done it? (reply: 4)
5074. genotyping transgenic mice with PCR - (reply: 1)
5075. High fidelity RT-PCR enzymes - looking for choices... (reply: 5)
5076. question about AmpliTaq - (reply: 2)
5077. Purification of Double stranded radiolabelled DNA - Purification problems (reply: 3)
5078. non-radioactive probel labeling options for library screening - (reply: 1)
5079. expression vector pHM6's map - (reply: 3)
5080. de-phosphorylating a vector - (reply: 7)
5081. Unstable plasmid ?? - Deletions only after ligation ! (reply: 2)
5082. Help! E.coli GM48: digestion/DNA degradation - (reply: 2)
5083. Failed to amplify a 2.1 kb PCR product - (reply: 3)
5084. mat agar dish and a cloning problem - (reply: 4)
5085. RNAse protection assay - No bands (reply: 2)
5086. Reuse of PCR purification column - (reply: 1)
5087. The definition of a real gene? - (reply: 3)
5088. BAC cloning problems - (reply: 2)
5089. RNA cleaning - (reply: 6)
5090. Problems isolating plant genomic DNA - (reply: 8)
5091. Help! Three way ligation including blunt-end oligo - (reply: 2)
5092. The amount of plasmid for ligation reaction - Help (reply: 2)
5093. blunt end ligation problems - (reply: 6)
5094. Agrose gel electrophoresis of bacterial RNA - 23srRNA and 16srRNA were not separated clearly (reply: 1)
5095. About PfuTurbo® DNA Polymerase and Pfu DNA Polymerase - (reply: 1)
5096. TA cloning - (reply: 5)
5097. Problem with DNase treatment of RNA for RT-PCR - (reply: 1)
5098. transfection of single copy of plasmid per cell - (reply: 3)
5099. storage of the homemade Taq polymerase - troubles with taq storage (reply: 1)
5100. Mastermix concept - (reply: 3)

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