1471. NaOAc versus MgOAc? - protein extraction (reply: 1)
1472. EMSA Supershift question - Bigger signal but no supershift (reply: 2)
1473. PPAR-gamma - (reply: 2)
1474. what affects primer specificity? - (reply: 2)
1475. Cloning problem: only frameshift mutants survive - (reply: 13)
1476. Assembly PCR - Design of PCR program for primers w/low Tm? (reply: 2)
1477. ampcillin sodium - (reply: 3)
1478. non-specific binding in RNA in situ hybridization - (reply: 5)
1479. Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
1480. mouse U6 promotor in human ES cells - (reply: 1)
1481. Primitive technique for preserving DNA - (reply: 1)
1482. modification of PCR buffers - When and how (reply: 1)
1483. plasmid prep with protein? contamination - (reply: 3)
1484. Templiphi amplification kit: How to determine the amount of DNA product - (reply: 2)
1485. RNA Storage - (reply: 8)
1486. Northern Blot - no specific bands - (reply: 2)
1487. Primers PCR - storage (reply: 5)
1488. what grade/brand of chemical? - (reply: 3)
1489. Dealing with VERY Mg sensitive PCR reactions - (reply: 4)
1490. qchip - (reply: 1)
1491. Plasmid extraction from filter paper - (reply: 3)
1492. how to ligate a gene repeatedly - (reply: 6)
1493. Is my DNA clean enough for a succesful ligation? - (reply: 15)
1494. What is the cause of my small E. coli colonies from transformation? - (reply: 7)
1495. As, MES - Storage of the solutions: As, MES (reply: 1)
1496. Serum Starvation - (reply: 2)
1497. Storage of B glycerophosphate NaF , Na3VO4, Na4P2O7 stock solution for lysis buf - How to store the protease kinase inhibitor soluble stock for lysis buf (reply: 3)
1498. plasmid - (reply: 4)
1499. defrosting........how do you do it? - (reply: 11)
1500. northern blot probe - is it necessary to remove unincorporated nucleotides? (reply: 4)

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