3241. Transformation using linearized plasmid? - (reply: 6)
3242. Primer concentration ? - (reply: 2)
3243. Stable transfection selection with puromycin - (reply: 2)
3244. PCR my cDNA & got nothing - (reply: 2)
3245. No Colonies on subcloning - (reply: 11)
3246. Multiple bands on RNA gel, why? - (reply: 6)
3247. Dimer of PCR products? - (reply: 2)
3248. prediction of transcription factor binding sites - which strand predictions are more important (reply: 1)
3249. Degradation of PCR product during purfication - Purification of PCR product for cloning (reply: 2)
3250. Interphase problems with TRIzol RNA extraction - And a question about reprecipitating RNA (reply: 5)
3251. RNA isolation from frozen tissue sections - Which Kit do YOU use? (reply: 3)
3252. Can DH5α be the host cell of the PCR-XL-TOPO from Invitrogen? - Help me (reply: 4)
3253. BAC clone amplification - (reply: 1)
3254. Problem ratio with RNA extract - (reply: 4)
3255. choice of reverse transcriptase - (reply: 4)
3256. Some DNA amount added cannot achieve reproducible in AP-PCR?! (urgent!) - (reply: 1)
3257. Nonspecific PCR amplification? - (reply: 2)
3258. My plasmid purity changes with different dilutions - (reply: 1)
3259. How to verify ancient DNA is not modern DNA? - (reply: 3)
3260. QIAquick PCR for preparing for restriction digestion - very low concentrations (reply: 4)
3261. Vector searching - (reply: 1)
3262. pcr products wont give me colonies - (reply: 3)
3263. Non-directional cloning - (reply: 5)
3264. Nested allele-specific PCR - (reply: 1)
3265. exons alternatively spliced - How to detect which variant is present? (reply: 4)
3266. No PCR product for genotyping - (reply: 2)
3267. lamda DNA digested with HindIII - (reply: 1)
3268. Micwowave technique for DNA extraction - (reply: 1)
3269. Problems with DNA Extraction of Tomatoes - DNA spooled is not clear and translucent (reply: 7)
3270. check plamid identity after miniprep - (reply: 5)

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