421. Transcript variation - measuring by RT-PCR - (reply: 3)
422. Primer analysis sites - Any suggestions? (reply: 3)
423. Problem with promoter PCR and cloning - (reply: 3)
424. DNA blunting - DNA blunting troubleshoot (reply: 4)
425. RAPD and viruses - RAPD using very very short primers (reply: 4)
426. Identification of weakly expressed novel gene - (reply: 1)
427. pIRES-EGFP - (reply: 2)
428. TA Cloning - TA cloning (reply: 2)
429. Large DNA fragmnet ampification - (reply: 6)
430. Cell growth inhibition by lipofectamine 2000 - (reply: 1)
431. Long Range PCR - (reply: 8)
432. please help me to be sure from design primer have it - (reply: 3)
433. Methyltransferase specificity - (reply: 1)
434. reverse-transcriptase PCR (internal control?) - (reply: 6)
435. QIAquick PCR purification kit for in vitro transcription? - (reply: 6)
436. Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
437. RNA gel - too many bands?? (reply: 3)
438. Agrobacterium miniprep problem - (reply: 4)
439. Luciferase reporter assay - (reply: 2)
440. LR PCR and running gels - Roche Expand Long Range dNTPack (reply: 5)
441. Improved electrophoresis of nucleic acids. - (reply: 15)
442. separating protein and nucleic acids - problem separating protien and nucleic acids (reply: 8)
443. When is the right time to adjust RNA concentrations for Rt-PCR? - (reply: 2)
444. 16s RNA of metagenomic DNA - soil bacteria (reply: 5)
445. HIV-pCL-CG - problem w/ bacteria - (reply: 1)
446. checking mRNA expression by reverse transcriptase PCR - (reply: 2)
447. Digest with BglII and SalI - (reply: 1)
448. DNA migration problem - Genomic DNA (reply: 1)
449. bacterial DNA isolation - various methods (reply: 1)
450. how to clone my insert DNA into vector? - (reply: 11)

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