3871. Amplify 4.5 kb pcr product without using special polymerase - (reply: 10)
3872. PCR master mix - (reply: 6)
3873. RECOMBINEERING - (reply: 1)
3874. SOS! asymmetric PCR, no PCR product - (reply: 2)
3875. Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
3876. degenerate PCR vs low stringency PCR - (reply: 1)
3877. how to mutant the gene on the genomic DNA? - (reply: 9)
3878. RT-PCR- help - Polyacrylmide Gel electrophrosis for PCR products (reply: 3)
3879. poly (dI-dC) - poly (dI-dC) role? (reply: 1)
3880. CsCl gradient - (reply: 1)
3881. No DNA on gel, but get PCR product - (reply: 2)
3882. What media would promote the best E.coli growth at a low cost for fermentation? - (reply: 18)
3883. handling cDNA - care in handling (reply: 1)
3884. explanation of miniprep of E. coli - miniprep of e.coli genomic dna? (reply: 1)
3885. Protocol for DNase I treatment of RNA - (reply: 4)
3886. PCR primer: 2 restriction sites? - (reply: 4)
3887. determine the molecular mechanisms for gene regulation - (reply: 1)
3888. Weird Band gel migration - Molecular biology (reply: 7)
3889. No PCR Product - Fungal Genomic PCR (reply: 9)
3890. E.coli not growing in liquid LB, but grows well on Agar plates - (reply: 22)
3891. primer orientation - (reply: 1)
3892. Annealing buffer for DNA oligos - RNA interference (reply: 2)
3893. Which Analysis of Ristriction sites on the DNA Seq should I believe? - help!!! I am driven mad by the result! (reply: 6)
3894. pCIS expression vector - (reply: 2)
3895. Degenerate primers for nested PCR? - (reply: 1)
3896. allelic variants in a population - genomic DNA that was used for Human genome project (reply: 3)
3897. Problems with Trizol - (reply: 15)
3898. Generation/Transformation into DY380 cells - (reply: 4)
3899. DNA extraction Ecoli strains - (reply: 3)
3900. DNA quantitation - (reply: 3)

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