571. Ligation failure - (reply: 1)
572. Maxiprep problems - (reply: 4)
573. PCR Inhibition by DNA? - (reply: 5)
574. Proof-reading and Taq mix - (reply: 3)
575. CaPO4 transfection and Temperature - (reply: 1)
576. RNA gel loading buffer? - (reply: 5)
577. is cDNA sequencing tricky? - (reply: 1)
578. RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
579. The best way for RNA extraction from cells in RNAlater? - TRI Reagent or RNeasy or something else? (reply: 1)
580. 3 piece ligation - (reply: 2)
581. qPCR standard curve problem - (reply: 1)
582. pGL3 problem - HELP!!!! (reply: 4)
583. Questions in collection and preservation of plant samples - Purpose: DNA Extraction for DNA fingerprint (reply: 5)
584. Strange results with DNase - any explanation? - (reply: 3)
585. Total RNA isolation for RT-PCR - (reply: 3)
586. Endofree DNA precipitation accident-- can I salvage my dna? - (reply: 1)
587. Primer design - Primer design for PCR with TE sites (reply: 2)
588. Isolating RNA from frozen tissues kept in vials - (reply: 4)
589. primer conditions for expression work - (reply: 5)
590. TOP10 competent cell with another vector - (reply: 1)
591. Possible for standards to run differently from samples? - (reply: 5)
592. best way to extract HIV RNA - Trizol vs Qiagen (reply: 2)
593. Can phenol be mixed with QG buffer from Qiagen Kit - (reply: 7)
594. kozak sequence - (reply: 4)
595. Cesium-cleaned environmental DNA - Help! - Running into problems after extracting ethidium (reply: 6)
596. loxP sites - (reply: 1)
597. GEL Extraction (any advanced techniques ?) - (reply: 7)
598. in silico search for transcriptions factors - (reply: 2)
599. chromatin immunoprecipitation - (reply: 2)
600. How to do mutations & rescue experiments - (reply: 1)

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