271. How to: protein-DNA binding in E. coli - (reply: 2)
272. qiagen dna purification columns - are they all the same? (reply: 2)
273. Help with non-radioactive EMSA! - unlabeled oligo causes bands to appear instead of disappear!!! (reply: 4)
274. Erroneous PCR product due to plasmid DNA contamination - (reply: 2)
275. buffer preparation calculations - (reply: 3)
276. Difference between TBE- and Denaturing Agarose Gel Electrophoresis of RNA - Gel for RNA (reply: 2)
277. reprecipitate DNA - reprecipitate DNA (reply: 11)
278. Protein purification issues - (reply: 4)
279. Primer design and degeneracy - Are synthesized sets identical? (reply: 2)
280. Orientation of insert - (reply: 3)
281. p53 protein - design experiments to illustrate p53 protein character. (reply: 2)
282. How long can I store RNA at -20C? - (reply: 2)
283. DynaBeads - mixing procedure (reply: 1)
284. alternative splicing. - (reply: 2)
285. high fidelity PCR polymerase - ask for help! (reply: 3)
286. B-catenin pathway - Lef/TCF-sensitive reporter gene assay (reply: 4)
287. exogenous and ectopic protein expression - what's the difference (reply: 2)
288. primers amplify additional band (!) - (reply: 1)
289. PCR Genotyping Problem - (reply: 7)
290. Electrophoresis RNA - (reply: 1)
291. resuspended primers in water - is this a problem?! (reply: 5)
292. plasma membrane tag - (reply: 2)
293. the mystery of the haunted pcr - Did my new preps degrade? Did my PCR fail? Or something more sinister? (reply: 5)
294. BL21 Expression - Transformation in BL21 (reply: 2)
295. time of DNase I treatment - (reply: 4)
296. Cleaning up RNA after double enzymatic reaction - (reply: 3)
297. Filtration of Acrylamide Solution - (reply: 1)
298. Is there a way to extract RNA and protein from the same sample? - (reply: 2)
299. A way to avoid insoluble precipitate with sodium acetate - (reply: 9)
300. plese help with paper - strange results with MCF7 and GADD45A (reply: 4)

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