991. PCR faint bands under UV light - (reply: 10)
992. How to construct a Double mutant - (reply: 7)
993. One of these days - Is this a critical mistake - reverse transcription (reply: 2)
994. Storing tissue for protein and RNA extraction? - (reply: 13)
995. PCR Help - 2kbp product - (reply: 12)
996. blue/white selection - (reply: 4)
997. RNA extraction from seawater filters-welcome to the challenge - (reply: 2)
998. Amount of Cell Lysate for Western Blot - (reply: 2)
999. Help-How is the RNA quality? - (reply: 3)
1000. Vector concentration following maxiprep - (reply: 4)
1001. Cloning Problem ! - (reply: 6)
1002. How to get rid the restriction sites in a plasmid ? - (reply: 5)
1003. Phenol Removal of DNA Preparation - (reply: 1)
1004. Expression vector for Pseudomonas - expression of a 10Kb gene in Pseudomonas aeruginosa (reply: 2)
1005. lots of colonies on media with ampiciline - (reply: 3)
1006. gene from 4-TOPO to expression vector - (reply: 1)
1007. PCR product of unlinearized plasmid - (reply: 1)
1008. Trizol for RNA extraction - (reply: 4)
1009. MoBio Plasmid Purification and Gel Extraction Kits - Problems with MoBio Kits (reply: 3)
1010. Chip Assay - PCR step (reply: 3)
1011. why low spins recommended in RNA precipitation trizol protocol? - Gibco, later Invitrogen recommend only max 12000g - why? (reply: 2)
1012. RNA degradation after DNase1 treatment. - RNA degraded after DNase1 treatment. Any idea? (reply: 1)
1013. Can I store bacteria cells at +4C before plasmid isolation? - (reply: 3)
1014. second strand cDNA synth without primer? - (reply: 2)
1015. Problems with site directed mutagenesis - (reply: 3)
1016. General question about restriction sequences in PCR primers - (reply: 3)
1017. SEVERE PROBLEMS WITH BUCCAL SAMPLES - (reply: 2)
1018. pulsed field gel electrophoresis of genomic DNA - (reply: 1)
1019. Multiple recombinant plasmids in transformed E Coli? - (reply: 2)
1020. Will TOPO TA cloning work on "old" purified PCR product? - (reply: 4)

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