3181. PCR result: Nothing! - Urgent! (reply: 2)
3182. Making competent E.coli is getting me desperate - After succeeding once, all tries after keep failing (reply: 13)
3183. pEBB or pEBG vector maps - (reply: 2)
3184. DNA extraction of agrobacterium, i need help - (reply: 1)
3185. RNA extraction without using liquid nitrogen - Can it be done? (reply: 3)
3186. can we add the terminaton codon in the 3' primer? - (reply: 1)
3187. KpnI and BamHI double digest help - (reply: 6)
3188. How many genes in Arabidopsis thaliana ? - (reply: 2)
3189. Size of 23s and 16s RNA different - (reply: 3)
3190. PCR probes for Southern blot - (reply: 1)
3191. Smear in PCR - (reply: 5)
3192. PCR amplification - (reply: 2)
3193. Maintaining organellar genomes - What's the force? (reply: 1)
3194. Site directed mutagenesis - wild type clone (reply: 4)
3195. Cloning - intronic sequences needed? - (reply: 4)
3196. Specific Specificity! - abt L/D- AAs ,D/L-sugars & all... (reply: 1)
3197. Primer dimers?Contaminations? - Unexpected bands! (reply: 5)
3198. hybridizazion - southern (reply: 1)
3199. reverse primer design with restriction site - (reply: 6)
3200. Tissue sample storage - (reply: 1)
3201. Denaturing Agarose gel for RNA - (reply: 10)
3202. BRAIN RNA EXTRACTION! - (reply: 3)
3203. Unstable clones - (reply: 3)
3204. how does dsDNA probes work in Northern ? - its a very basic qn but i am confused (reply: 5)
3205. glass milk for DNA cleanup? - (reply: 1)
3206. Is it obligatory to precipitate DNA from ligation before transformed into E.coli - (reply: 7)
3207. southern again - transfert (reply: 4)
3208. southern - loading DNA (reply: 2)
3209. Expression analysis - (reply: 1)
3210. Different RNA sources, Different PCR optimal condition? - (reply: 5)

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