511. mRNA sequence - (reply: 2)
512. Non-directional cloning - (reply: 1)
513. Cloning from genomic DNA - (reply: 7)
514. need to identify domain of my sp1 transcription factor - sp1 domain (reply: 1)
515. HELP- Can I save my RNA now? - RNAse free pipette tips (reply: 7)
516. GenomiPhi first-strand cDNA - Can GenomiPhi be used on single-stranded DNA? (reply: 2)
517. Promoter cloning... - Problems (reply: 3)
518. how to improve PCR yield? - (reply: 5)
519. PCR a fragment of 6kb - (reply: 14)
520. Blunt end ligation - (reply: 8)
521. How to pull out one stranded DNA binded with histon and conserve another one? - (reply: 1)
522. FACS sorting into what solution for RNA isolation? - (reply: 2)
523. frustrating maxiprep problem - (reply: 4)
524. How to pull out bottom string DNA and reserve up string DNA - (reply: 2)
525. help on directional cloning - (reply: 3)
526. PCR Performance Test - QC test for new Taq Polymerase (reply: 3)
527. DNA gel electrophoresis problem? - (reply: 5)
528. EXOSAP-IT degrades my PCR products - (reply: 3)
529. Problem with drying down polyacrylamide gel - (reply: 4)
530. high 260/280 ratio after maxiprep - (reply: 2)
531. Do I have to pick signle colony in such a case? - (reply: 4)
532. pkD46 Recobining with itself - (reply: 3)
533. primer design - (reply: 2)
534. Tool for complementary restriction enzymes? - REs producing overhangs to religate (reply: 5)
535. help with degenerate primer design - (reply: 1)
536. spatial and temporal patterns of transcription - (reply: 3)
537. PCR: Big Primers = Big Problems (for me) - I'm really not sure what's going on in those poor little tubes... (reply: 2)
538. Mitochondrial isolation and storage. - How do I store (reply: 2)
539. Phenol-Chloroform Extract vs Commercial Kits - (reply: 7)
540. RNA precipitation with DNase treatment - (reply: 1)

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