4591. Co Precipitant - Glycoblue Ambion? - (reply: 3)
4592. Can CIP dephosphorylate 3' protruding end? - (reply: 3)
4593. samples are obtained from in vitro or in vivo? - (reply: 1)
4594. "cDNA must accurately represent RNA input amounts" - (reply: 1)
4595. cDNA amount - (reply: 1)
4596. DNA concentration for ligation - (reply: 8)
4597. PCR primer Tm and concentration question - (reply: 3)
4598. Definition of error using LightCycler??? - (reply: 3)
4599. antibiotics concentration for JM109 and DH5 alpha - (reply: 3)
4600. Maximum insert size in a pGEM Teasy vector - (reply: 2)
4601. help on transformation - BAC clones transformation (reply: 4)
4602. colony formation assay - (reply: 3)
4603. problems with cloning-transformation - (reply: 2)
4604. reverse-transcribed RNA - (reply: 1)
4605. transformation problems - (reply: 4)
4606. internal control in semi quantitative rt pcr - question (reply: 2)
4607. PKC translocation - how to detect PKC translocation (reply: 2)
4608. How to avoid secondary structure in primer - (reply: 2)
4609. Amont of DNA to use for transient Transfection?? - (reply: 2)
4610. cDNA - (reply: 4)
4611. Reverse Transcription - (reply: 2)
4612. Alternative to phenol-chloroform purification of DNA - Genomic DNA purification (reply: 4)
4613. in-vitro transcription - (reply: 4)
4614. How to determine appropriate agarose % - (reply: 8)
4615. High OD260/OD280 ratio - (reply: 1)
4616. How to get the sequence detected in Northern blot? - (reply: 1)
4617. DNA sonication !!! - (reply: 1)
4618. PCR reamplifying problem - (reply: 5)
4619. transport RNA and cDNA - (reply: 2)
4620. Cloning a gene without the sequence of the target species - (reply: 3)

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