5431. Smear after second PCR - (reply: 2)
5432. Problematic ligation - problems with ligation (reply: 1)
5433. CIAP absolutely required? - (reply: 3)
5434. still more about cloning PCR products - (reply: 9)
5435. Restriction Digest - NarI-my very special friend! (reply: 4)
5436. single cell PCR - (reply: 8)
5437. Methylation and restriction digestion problem - (reply: 8)
5438. Annealing temperature for PCR - (reply: 3)
5439. poly(A)-RNA extraction from cells - (reply: 1)
5440. Invitrogen platinum pfx enzyme not so fantastic - problems with pcr product gel migration (reply: 1)
5441. inhibitor of glucose-6-phosphatase - help (reply: 1)
5442. DNA extraction - Is 2years ago extracted DNA still usable (reply: 2)
5443. PstI cutting - (reply: 3)
5444. Genomic DNA amplification - (reply: 4)
5445. Bands are like a "w" - (reply: 1)
5446. Ligation Reaction - (reply: 8)
5447. PCR works first time, then not! - (reply: 1)
5448. RT product check - Can I qualify my cDNA? (reply: 2)
5449. large fragment cloning - (reply: 2)
5450. wierd plasmids: maxi-prep never work - (reply: 13)
5451. Banding in agarose gel wells. - amplified fragment will not migrate! (reply: 7)
5452. A question about adenovirus infection 293 cells - (reply: 5)
5453. Cloning long fragments - (reply: 18)
5454. a question about mutagenesis - (reply: 5)
5455. RT-PCR, why two PCR steps? - two PCRs after reverse transcription?? (reply: 2)
5456. Annealing Temperatures - RT-PCR (reply: 1)
5457. total rna extraction from whole blood - for rt pcr (reply: 3)
5458. Does phenol affect Norhern blot? - need your advice (reply: 3)
5459. mRNA of bacteria - (reply: 1)
5460. DNA arrays for low gene expression ? - (reply: 1)

© 1999-2008 Protocol Online, All rights reserved.