2131. Thymus DNA extraction - (reply: 3)
2132. Cloning a promoter sequence from genomic dna - (reply: 3)
2133. Clone 2kb and 5 kb inserts into 6kb vector - Which order and vector:insert ration? (reply: 3)
2134. PCR detectable units - PDU (reply: 2)
2135. designing of probes - (reply: 5)
2136. maxiprep troubleshoot - (reply: 15)
2137. Heat shock proteins - Any test kits which will show the presence or otherwise (reply: 2)
2138. pcDNA3.1 /V5 His Topo - (reply: 4)
2139. dna recovery from gel - (reply: 7)
2140. Designing forward primer incorporating Nde1 cut site - Tips on cloning with Nde1? (reply: 2)
2141. about ligation problem - insert and vector size (reply: 9)
2142. Manual Dot Blot and SA-HRP detection Protocol...HELP - (reply: 3)
2143. no results in RACE PCR - (reply: 5)
2144. HELP! In-vitro transcription results only in smear! - (reply: 2)
2145. RNA re-extraction with phenol - I can't get a good RNA quality (reply: 3)
2146. EMSA Problem - (reply: 7)
2147. Very resistand band in PCR - (reply: 7)
2148. Nonradioactive overgo probes - (reply: 2)
2149. Can I use AMP plates with Agrobacterium? - (reply: 1)
2150. small size DNA isolation - (reply: 1)
2151. no DNA band - (reply: 4)
2152. G+A Ladder - (reply: 2)
2153. M13 phage pfu determination - (reply: 1)
2154. Do you grow bacteria at 37°Cafter heat shock, before plating? - (reply: 8)
2155. No bands in RFLP - (reply: 2)
2156. which -20 or -80 is more less degradation of cell lysates - (reply: 4)
2157. Ligation at 16°C,ON: where to keep it till transformation? - (reply: 4)
2158. Visualising 200-400+ kDa proteins by gel electrophoresis - (reply: 3)
2159. Nla III digestion - (reply: 2)
2160. Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)

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